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cy5 donkey anti goat  (Jackson Immuno)


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    Structured Review

    Jackson Immuno cy5 donkey anti goat
    Cy5 Donkey Anti Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 2750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+goat+cy5/Cy+3+AffiniPure+Donkey+Anti-Rabbit+IgG/pmc13020104-287-49-52
    Average 96 stars, based on 2750 article reviews
    cy5 donkey anti goat - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Enhancing myelinogenesis through LIN28A rescues impaired cognition in PWMI mice.
    Article Snippet: Regular immunostaining was performed using primary antibodies to oligodendrocyte transcription factor 2 (Olig2, Abcam, ab109186, 1:1000), platelet-derived growth factor receptor alpha (PDGFRα, R&D Systems, AF1062, 1:1000), myelin-associated glycoprotein (MAG, Santa Cruz, sc-376145, 1:500), and Ki67 (Abcam, ab16667, 1:1000) overnight at 4 oC. .. Sections were then incubated with secondary antibodies donkey-anti-rabbit Cy2 (Jackson ImmunoResearch, 711-225-152, 1:1000), donkey-antimouse Cy3 (Jackson ImmunoResearch, 715–165-151, 1:1000), donkey-anti-goat Cy5 (Jackson ImmunoResearch, 705-175-147, 1:1000) for 2 h at room temperature (25 °C). .. After staining, sections were mounted on slides with Fluoromount-G anti-fade medium containing DAPI (SouthernBiotech).

    Article Title: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines.
    Article Snippet: .. Following incubation in secondary antibody (1:200 donkey anti-goat Cy5, Jackson ImmunoResearch 705-175-147), RPE/choroids and retinas were washed 5 × 5 minutes in wash buffer. ..

    Article Title: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines
    Article Snippet: .. Following incubation in secondary antibody (1:200 donkey anti-goat Cy5, Jackson ImmunoResearch 705-175-147), RPE/choroids and retinas were washed 5 × 5 minutes in wash buffer. ..

    Article Title: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines
    Article Snippet: Slides were blocked (PBS with 0.05% Triton X-100, 2% Bovine Serum Albumin [BSA] and 2% normal donkey serum [NDS]) and then incubated overnight at 4°C in 1:100 goat anti-tdTomato (LS Bio, LS-C340696) and 1:100 mouse anti-RPE65 (Novus Biologicals, NB100–355). .. Following three 5-minute PBS washes, slides were then incubated for 2 hours at room temperature with corresponding conjugated secondaries (1:200 donkey anti-goat Cy5 [Jackson ImmunoResearch 705-175-147], and 1:200 donkey anti-mouse Cy2 [Jackson ImmunoResearch 715-545-150]) and 1:1000 DAPI. tdTomato was detected via direct fluorescence (native signal) and indirect detection. .. As a negative, control an additional slide was stained with anti-goat IgG isotype control (ThermoFisher Scientific 31245), 1:100 mouse anti-RPE65 (Novus Biologicals NB100–355), and 1:1000 DAPI.

    Article Title: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines.
    Article Snippet: Slides were blocked (PBS with 0.05% Triton X-100, 2% Bovine Serum Albumin [BSA] and 2% normal donkey serum [NDS]) and then incubated overnight at 4°C in 1:100 goat anti-tdTomato (LS Bio, LS-C340696) and 1:100 mouse antiRPE65 (Novus Biologicals, NB100–355). .. Following three 5-minute PBS washes, slides were then incubated for 2 hours at room temperature with corresponding conjugated secondaries (1:200 donkey anti-goat Cy5 [Jackson ImmunoResearch 705-175-147], and 1:200 donkey anti-mouse Cy2 [Jackson ImmunoResearch 715-545-150]) and 1:1000 DAPI. tdTomato was detected via direct fluorescence (native signal) and indirect detection. .. As a negative, control an additional slide was stained with anti-goat IgG isotype control (ThermoFisher Scientific 31245), 1:100 mouse anti-RPE65 (Novus Biologicals NB100–355), and 1:1000 DAPI.

    Fluorescence:

    Article Title: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines
    Article Snippet: Slides were blocked (PBS with 0.05% Triton X-100, 2% Bovine Serum Albumin [BSA] and 2% normal donkey serum [NDS]) and then incubated overnight at 4°C in 1:100 goat anti-tdTomato (LS Bio, LS-C340696) and 1:100 mouse anti-RPE65 (Novus Biologicals, NB100–355). .. Following three 5-minute PBS washes, slides were then incubated for 2 hours at room temperature with corresponding conjugated secondaries (1:200 donkey anti-goat Cy5 [Jackson ImmunoResearch 705-175-147], and 1:200 donkey anti-mouse Cy2 [Jackson ImmunoResearch 715-545-150]) and 1:1000 DAPI. tdTomato was detected via direct fluorescence (native signal) and indirect detection. .. As a negative, control an additional slide was stained with anti-goat IgG isotype control (ThermoFisher Scientific 31245), 1:100 mouse anti-RPE65 (Novus Biologicals NB100–355), and 1:1000 DAPI.

    Article Title: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines.
    Article Snippet: Slides were blocked (PBS with 0.05% Triton X-100, 2% Bovine Serum Albumin [BSA] and 2% normal donkey serum [NDS]) and then incubated overnight at 4°C in 1:100 goat anti-tdTomato (LS Bio, LS-C340696) and 1:100 mouse antiRPE65 (Novus Biologicals, NB100–355). .. Following three 5-minute PBS washes, slides were then incubated for 2 hours at room temperature with corresponding conjugated secondaries (1:200 donkey anti-goat Cy5 [Jackson ImmunoResearch 705-175-147], and 1:200 donkey anti-mouse Cy2 [Jackson ImmunoResearch 715-545-150]) and 1:1000 DAPI. tdTomato was detected via direct fluorescence (native signal) and indirect detection. .. As a negative, control an additional slide was stained with anti-goat IgG isotype control (ThermoFisher Scientific 31245), 1:100 mouse anti-RPE65 (Novus Biologicals NB100–355), and 1:1000 DAPI.

    Staining:

    Article Title: Lymphatic Activation of ACKR3 Signaling Regulates Lymphatic Response After Ischemic Heart Injury
    Article Snippet: .. Immunofluorescent staining was performed using the following primary and secondary antibodies: rabbit anti-LYVE1 (lymphatic vessel endothelial hyaluronan receptor 1; 1:200, 70R-LR005; Fitzgerald), goat anti-CD45 (1:50, NB10077417; Novus Biologicals), goat anti-F4/80 (1:50, 565409; BD Pharminogen), donkey anti-rabbit Cy3 (1:200, 711-165- 152; Jackson ImmunoResearch), and donkey anti-goat Cy5 (Cyanine 5; 1:200, 705-175-147; Jackson ImmunoResearch). ..



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